The Kodak camera allows one to obtain quantitative measurements of mass or molecular weight from stained gels, and to include photographs of gels in publications.

The Kodak can capture images of:

  • Plasmids, PCR products, Restriction fragments
  • RNA
  • Proteins

The system consists of:

  • a Kodak DC-120 digital camera
  • 1.2M pixel resolution and a
  • 3x zoom lens,
  • hoods, filters, and UV and visible-light transilluminators (for use with including ethidium bromide, silver, coomassie, and other stained gels.)
  • a networked Macintosh "G3" computer
  • a networked 1200x1200 dpi laser printer
  • a zip drive

CALIBRATED MOLECULAR WEIGHTS:

If your gels include one or more lanes of molecular weight standards, the software will use those to obtain a calibrated molecular weight of unknown bands in the gel.

MASSES AND CONCENTRATIONS: If you include one or more mass-standards in the gel (i.e., a band containing a known amount of a nucleic acid or protein), the software will provide quantitative mass measurements of unknown bands, from the relative integrated intensities. With a known loading-volume, these data provide quantitative concentration measurements of specific species (e.g., one particular protein or restriction fragment) even in a complex mixture.

DIGITAL PICTURES: .Images can be saved in PICT or TIFF formats for use with programs such as Adobe Photoshop, etc.

WHAT ARE SOME ALTERNATIVES at the KECK FACILITY?

  • Storm 860 phosphorimager / fluorimager system
  • HPLC system
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