Quantitative Real-Time PCR
Key features of the iCycler system include:
- Presently 2 emission/excitation filter sets for the following fluorescent dyes
(upgradeable with up to 4 filters sets within wavelength range of 400-700nm):
- Fluorescein (FAM) and SYBR Green I
- Texas Red and ROX
- Monitoring of up to 96 wells with up to 4 fluorophores per well simultaneously
- Dynamic range of at least six orders of magnitude for detecting specific sequences with excellent sensitivity and precision
- Calibrated with the Bio-Rad iQ 96-Well PCR Plates to a 25 μl sample volume and the optical quality sealing tape
- Melt Curve Analysis tool to confirm the specificity of primers as well as reveal the presence of primer-dimers
- Beacon Designer Software for use in designing probes for molecular beacons and TaqMan® and enables BLAST searches, Primer Design and Data Management