Analytical Ultracentrifuge (XLA)
Analytical ultracentrifugation is a powerful biophysical technique that
can be used to answer a large range of questions frequently encountered
in modern biochemical and molecular biophysical research
Examples of biological
problems suitable for AUC Analysis
|
·
Purity
Assessment |
·
Binding
Stoichiometry |
|
·
Mutation Effects |
·
Self-Association behavior |
|
·
Molecular Weigh Determination |
·
Conformational Changes and Effects |
The Beckman XLA -70 instrument can measure absorbance in the UV-Vis range. The samples should have absorption between .1 - 1 OD at the chosen experimental wavelength
The Keck Facility has two rotors. The 4-hole rotor holds 3 cells and can be used for velocity experiments (2-sector cells, each cell holds one sample and one reference solution) and equilibrium experiments (2 or 6-sector cells, which hold 3 samples and 3 reference solutions). The 8-hole rotor will in general only be used for equilibrium experiments, allowing one to run 21 samples at the same time when using the 6-sector cells (7 cells, each with 3 samples and 3 reference solutions)
Service Options offered by the Keck Facility:
Level 1
( Full project) (Experimental Design, Data Acquisition and Data
Analysis)
Level 2
(Experimental Design and Data Acquisition)
Level 3
(Data Acquisition)
Level 4
(Data Analysis)
Level 5
(Self Service – Level 6 training required)
Analytical Ultracentrifgation Training Services:
Level 6
(Instrument Training)
Level 7 data analysis (AUC/UltraScan Workshop)
Please contact us for more information on the AUC services
Location: Cook Hall, 4106
FOM ID: XLA_AUC