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The Northwestern University RNAi Core provides researchers with the latest in whole genome RNAi collections. With the rapid rate of scientific discoveries being made in RNAi, this Core Program collaboration with Open Biosystems allows for rapid expansion and continued institution access to Open Biosystems evolving RNAi product lines. This core has obtained the RNAi lenti-viral libraries for both human and mouse (currently 120,000 total clones), and also provides the research community with the required expertise to manage the libraries and execute suitable screens.
The microRNA-adapted shRNA (shRNAmir) libraries were developed
in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard).
shRNAmir triggers for mammalian RNAi are based on current knowledge of
the endogenous microRNA processing pathway, with constructs designed to
mimic the natural microRNA primary transcript, thereby enabling specific
processing by the endogenous RNAi pathway and producing more effective knockdown.
In addition, each gene-specific shRNAmir sequence has been selected based
on thermodynamic criteria for optimal small RNA performance.
Click here to
access information about the RNAi Library (Northwestern University researchers
only)
Click here to access
information about Sigma's RNAi services
Email Northwestern
University RNAi Core
Additional information about whole genome RNAi collections may
be found at:
Open Biosystems shRNAmir resources
RNAi Codex at Cold Spring Harbor
Viral Vector Translational Resource Center at Children's Memorial Research Center (CRMC)
Please visit the other Library Screening pages:
