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Yeast TAP collection -The Yeast-TAP-Fusion Library allows the purification and selection of the entire yeast proteome and associated components using two simple affinity selection steps in tandem, enabling the development of a range of high-throughput functional assays. Click here for more info. Yeast GFP collection -The GFP-tagged yeast strain collection and database covers three-quarters of the S. cerevisiae proteome and over two-thirds of previously unlocalized proteins. The collection provides a tool for analyzing the response to specific external stimuli or growth conditions and can also be used to assay the effects of deletion or mutation of a protein of interest on the localization of a particular protein. Click here for more info. Yeast GAL-GST library - The GAL-GST library is over-expression library covering 85% of all yeast genes. The over-expression library enables broad examination of gain-of-function phenotypes. Each strain carries a yeast ORF tagged at its amino terminus with GST and expressed from the inducible GAL1/10 promoter. Click here for more info. Yeast Two Hybrid Array - The yeast two-hybrid library contains an array of yeast ORFs fused to the GAL4 activation domain and transformed into Mat-A cells. The array can be mated to Mat-alpha cells containing a bait peptide fused to the GAL4 DNA binding domain. The reporters for this screen are HIS3, ADE2 and LacZ. Click here for more info. Yeast Deletion Libraries - Four deletion collections are available; knockouts of the 'a', 'alpha', homozygous diploid and heterozygous diploid strains. Each Yeast Deletion represents a unique gene-knockout of the S. cerevisiae genome. Click here for more info.
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